Puren Solutions UVC Light Products
UVC LIGHT
FOR MICROBIAL MANAGEMENT

WHAT IS UVC LIGHT AND WHAT DOES IT DO?

UVA and UVB are more commonly known bands of the ultraviolet spectrum. They are emitted by the sun and the reason we have sunscreen. These bands are also manufactured in man-made devices such as tanning beds, black lights, UV therapy lights, etc. The greatest concern surrounding UV wavelengths is damage to skin and eyes. This occurs through long-term, unprotected exposure. Ultraviolet-C, or UVC, is also emitted by the sun. However, it is not able to penetrate the ozone layer. As such, human exposure to UVC is always man-made (unless you are an astronaut). UVC irradiation refers to germicidal wavelengths of light in the 200-280 nm range.

UVC light works by deactivating and killing pathogens by damaging their DNA. The UVC spectrum, especially between 250-270 nm, is strongly absorbed by pathogens, making it the most lethal range for microorganisms. Not all UVC is equal in its germicidal capacity. UVC in the 222 nm range is indeed germicidal (and appealing due to it being considered safer for skin and eyes). However, the amount of time a pathogen needs to be exposed for deactivation and death is substantially greater than UVC in the 265 nm range. For industrial application, it is important to pay attention to UVC product specifications. This will ensure it can provide proper disinfection for the intended application.

IS UVC LIGHT SAFE?

As with many disinfection tools out there, UVC light can cause burns to skin and eyes if not used properly. It is not recommended to stare directly at the lights or hold them directly over your skin. Wearing safety glasses and clothing that covers your skin is recommended. Especially parts of the body that may have greater potential for exposure during use of UVC light devices.

A BRIEF HISTORY OF UVC

The germicidal effect of UV light has a long known history. It was first recognized for its germicidal effects in the late 1800s. Physiologist Arthur Downes and scientist Thomas P. Blunt first reported the inactivation of bacteria by sunlight. In 1893, Niels Finsen demonstrated that chemical rays (what we now call UV light) from sunlight or arc lamps had antibacterial effects. However, the exact mechanism for this effect was still speculatory. (Think of the open-air treatments in specially designed wards for patients battling the Spanish flu during the 1918-1919 pandemic.)

In 1903, J.E. Barnard and H. Morgan identified the UV spectrum around the 250 nm range as biocidal. Shortly thereafter, the study and publication of research around “ultraviolet radiation” grew rapidly. It was also during this time that the different categories of UV light were named – i.e. near UV, far UV, UVA, UVB, and UVC.

As research continued, major discoveries were made about its ability to destroy E. coli, tuberculosis, and measles. In the early 1990’s, UVC light became widely used – and still is today – in places like hospitals, schools, and airports.

UVC Light treatments by Finsen's UVC arc light

Niels Ryberg Finsen’s original UV arc light used to treat various skin conditions, including Lupus. Reprinted from DOI:10.2147/NDD.S26593

UVC FOR MICROBIAL MANAGEMENT

UVC light for microbial management offers a non-chemical approach to disinfection. Research has shown that UVC can effectively inactivate bacteria, viruses, fungus, and mold. There are many types of UVC light systems and equipment on the market. The use of lamps has a much longer history than that of LEDs. These lamp-based systems utilize UV lamps, fixtures, ballasts, and reflective surfaces. However, in recent years, LEDs have experienced rapid advancements in their technological development. This has opened the door for unlimited form factors and applications previously unavailable with lamps.

The most prominent applications of UVC light are in air, surface, and water disinfection. With advances in technology, more uses are being explored and implemented.

COMMON UVC AIR APPLICATIONS
COMMON UVC SURFACE APPLICATIONS
COMMON UVC WATER APPLICATIONS

Research into UVC light treatment for wound care is promising. Aided by the development of technological advancements – such as UVC LEDs – new applications previously inaccessible to UVC lamp devices are now being explored.

UV light sunset, two farmers walking in field
UVC APPLICATIONS IN AGRICULTURE

Numerous crops are impacted by molds, mildew, and bacteria. One example is in California, where powdery mildew is estimated to account for nearly 75% of total pesticide application by grape growers. In cost, this equates to 3-7% of their total gross production value. Likewise, national losses in wheat yield due to powdery mildew have been estimated to be almost as much as half the total crop harvest. One of the biggest challenges in combating these pests is the increase of chemical resistance.

UVC light disinfects plants in two ways: germicidal effects and biological responses. Research cites the efficacy of UVC light in managing disease through direct germicidal activity as well as indirect ability to induce defense responses in plants.

Depending on the plant/species, UVC has also been shown to up-regulate plant metabolites (decreasing need for growth regulators ), resulting in:

Research continues to build supporting UVC light as a powerful tool against prevalent agricultural woes. For example, multiple studies on multiple crop types revealed substantial decreases in plant and produce sensitivity to Botrytis and powdery mildew diseases. 

Research also shows a significant decrease in already present Botrytis and powdery mildew counts on plants and plant products – both in the field and post-harvest. In multiple studies, nighttime applications of UVC provided suppression of disease severity statistically equivalent to chemical standards with no harm to plants or fruits and no chemical residue. Like the use of pesticides/fungicides, care should be taken to ensure the safety of personnel applying UVC.

From seed to harvest to storage/transport, UVC light is proving a powerful tool for bolstering plant resistance, mitigating pathogen outbreaks, and reducing chemical usage.

UVC APPLICATIONS IN FOOD PROCESSING

According to estimates by the Food and Agriculture Organization (FAO) of the United Nations, one-third of food produced globally is lost or wasted each year. This includes losses at the different stages of the food supply chain – including on-farm, harvest, processing, storage, and transportation.

For over 50 years, UVC has been used in food processing to disinfect water, air, and surfaces. The US FDA’s HACCP, Health Canada, and European Union have already approved the use of UV light for pathogenic control in many food industries. UVC light’s efficacy in managing disease/pathogen mitigation through direct germicidal activity and defense responses is bolstered by research showing it does not compromise quality in many plant and food materials. These defense responses are important in resistance to spoilage agents.

Conventional food safety technologies (thermal processing & pasteurization, cold plasma processing, radiofrequency heating, pulsed electric fields, etc.) have been studied extensively for application in the food industry. But they often alter physical and nutritional properties of food. UVC light – especially from LEDs – offers a strong tool free of the undesirable characteristics of traditional food safety approaches.

The FDA Food Safety Modernization Act (FSMA) requires facilities to have microbial control plans. Common pain points lie in cleanliness of suppliers, human related factors, and how process control is being addressed. These plans help address these issues through regular implementation of a multi-step process:

Conditions commonly found in food processing facilities are excellent for microbial growth. Microorganisms have access to plenty of food, moisture, and ideal temperatures. Many studies have shown significant reduction of pathogens such as norovirus, E. coli, Salmonella, and Listeria on a range of food and food processing surfaces. For example, bacterial counts of listeria were significantly reduced (over 90% and up to 99% reduction) on multiple conveyor belt types using UVC light. Another study found up to 99.999% reduction of E. coli on four different conveyor belt materials (and egg surfaces) through the application of UVC light.

Studies also show the extension of shelf-life and successful control of post-harvest disease and decay in many food products. Primarily through the killing of spoilage organisms and up-regulation of plant/produce defense mechanisms. UVC also caused fewer changes (or no changes at all) in the nutritional and sensory properties of food compared to thermal processing.

Dosage, especially uniform dosing specific to the application, is a core component for successful UVC implementation. As UVC light technology continues to evolve and application research broadens, it is proving powerful potential for every step of control plans and for the food supply chain in general.

UVC APPLICATIONS IN HOSPITALITY

Hospitality is an industry that regularly interacts directly with customers, handles food, and has a wide range of shared spaces it must keep clean. From guest room disinfection to microbial mitigation of common touch points (doorknobs, keyboards, telephones, TV remotes, etc.) to laundry to food storage, preparation, and delivery, pathogen mitigation in hospitality is everywhere. The ramifications for failed sanitation protocols are great. They can lead to illness and death of customers, which lead to loss of trust and brand damage. All of which equate to financial losses.

Hospitality faces challenges with chemical exposures (think degreasers, oven cleaners, floor cleaners, a wide range of sanitizers, and even laundry soaps), property materials wear and tear, and food/food surface contamination. This is compounded by the tough and adaptable nature of bacteria, which increasingly develop resistance to chemical disinfection approaches.

Patrons are more aware and concerned with cleanliness than ever before. A 2020 study by the American Hotel & Lodging Association found cleanliness as guests top priority. Likewise, a study in 2018 found 81% of surfaces in hotels had some fecal bacteria present. These surfaces were not limited to bathrooms. Fecal bacteria was found on light switches, TV remotes, ice buckets, decorative bedding, and more.

For example, a study of bathroom counters in 4-star hotels were found to have an average of 2.5 million Colony Forming Units (CFU) per square inch. (For reference, the average home bathroom has 452 CFU per square inch.) Studies have also found that mops and cleaning cloths used by cleaning staff are often contaminated with germs while being used through a variety of spaces. One study planted a virus in a hotel room and was able to track its spread via hotel cleaning staff to three other rooms nearby. It can be expected, given the number of people moving through, that high levels of pathogens will be present in hospitality spaces. What is increasingly apparent is that current protocols are leaving far too many germs behind.

A study compared UVC light and alcohol wipes for routine once-daily disinfection of surfaces. It found that UVC reduced the number of housekeeping hours in half with similar pathogen reduction efficacy as the alcohol. Another study found a 94% reduction in pathogens when UVC light was added alongside the standard use of disinfectants.

Backed by extensive history and documentation of effectively killing microbes, UVC light offers powerful disinfection tools for just about any hospitality sanitation plan. Technological advancements are making it increasingly possible to deploy UVC in more settings than before with greater cost-effectiveness. It can easily be integrated into housekeeping processes, providing rapid sanitization of surfaces while reducing use of and exposure to chemicals. 

UVC LIGHT SOURCES: LAMPS VS LEDS

Historically, a range of mercury (low-, medium-, and high-pressure), xenon, and excimer lamps have been the source of man-made UVC light.

This past decade has seen incredible advances in UVC LED technology. The novelty around home UVC LED devices is intriguing. But a majority of these devices lack the necessary power and durability for commercial applications. Industrial-strength UVC light is now possible for more applications with LED technology. It can readily be added to the arsenal of tools (even replacing or minimizing the use of some) available to cleaning and maintenance teams. It also offers a reliable addition for consistency in process control.

UVC LEDs have specific advantages over UV lamps:

LEDs offer a compact light source that does not require an electronic ballast for starting.
LEDs can be utilized where space does not allow regular UV lamps.
LEDs do not use the toxic heavy metals found in UV lamps.
LED efficacy is not affected by contact with oils from skin.
LEDs can be arranged in a variety of arrays and packaging for optimal use and disinfection.
LEDs provide directional light, reducing wasted energy seen with 360 degree emission of light by UV lamps.
LEDs can produce UVC at optimal wavelengths (265 nm) for germicidal effectiveness.
LEDs offer a lower maintenance solution to UV lamps.

One of the most exciting and important aspects of UVC LEDs is the potential for endless form factors and customizations. Since UVC light is only effective for pathogens directly in its path, a downfall of the technology (conventional or LED) in automated applications is “shadowing.” This is easily overcome by the packaging flexibility of UVC LEDs.

LEDs open the door for UVC application in conditions and spaces not safe, practical, or feasible for lamps. LED packaging can be configured to allow the delivery of UVC directly to surfaces, products, tools, and equipment. This allows for site-specific management of hygiene and microbial risk. It is now possible to integrate UVC into automated processing lines without safeguards for bulb breakage or special consideration for exposure to cleaning cycles.

IS UVC RIGHT FOR ME?

Facilities that properly implement UVC light in their systems and processes – regardless of industry – have seen direct correlations between the application of UVC and increased profits. In many situations, it can:

REFERENCES

  1. Coughlin Insurance Services. (2023, March 5). The Cost of Food Recalls: 2022. https://coughlinis.com/the-cost-of-food-recalls-2022/
  1. Ortiz-Araque, L.C., Darré, M., Civello, P.M., & Vicente, A.R. (2022) Short UV-C Treatments to Extend the Shelf-Life of Fresh-Cut Strawberries (Fragaria x ananassa Duch cv. Camarosa). Ingeniería e Investigación, 42(3), e201. Epub October 27, 2022. https://doi.org/10.15446/ing.investig.88627
  1. Yuan, J., Wang, H., Li, Y., Chen, L., Zheng, Y., Jiang, Y., Tang, Y., Li, X., Li, J., & Wang, L. (2022). UV-C irradiation delays browning of fresh-cut “Fuji” apples. Journal of Food Processing and Preservation, 46, e16338. https://doi.org/10.1111/jfpp.16338
  1. Monteiro, M.L.G., Mársico, E.T., Mutz, Y.d.S. et al. Combined effect of oxygen-scavenger packaging and UV-C radiation on shelf life of refrigerated tilapia (Oreochromis niloticus) fillets. Sci Rep 10, 4243 (2020). https://doi.org/10.1038/s41598-020-61293-8
  1. LEDs Magazine. (2023, March 1). SETi and the USDA document UV LEDs extending shelf life of fresh produce. https://www.ledsmagazine.com/horticultural-lighting/agriculture-aquaculture/article/16697306/seti-and-the-usda-document-uv-leds-extending-shelf-life-of-fresh-produce
  1. Yuan, J., Wang, H., Li, Y., Chen, L., Zheng, Y., Jiang, Y., Tang, Y., Li, X., Li, J., & Wang, L. (2022). UV-C irradiation delays browning of fresh-cut “Fuji” apples. Journal of Food Processing and Preservation, 46, e16338. https://doi.org/10.1111/jfpp.16338
  1. Hemmaty, S., Moallemi, N., & Naseri L. (2006) Shelf-life and quality of apple fruits in response to posthartvest application of UV-C radiation. Journal of Applied Horticulture, 8(2): 114-116. https://www.horticultureresearch.net/jah/2006_8_2_114_116.PDF
  1. Malek, N.I., Abdullah, W.Z.W., & Sembok, W.Z.W. (2021) Effects of UV-C Radiation in Delaying Ripening of Berangan Banana (musa sp. AAA Berangan). University Malaysia Terengganu Journal of Undergraduation Research, 3(4): 173-182. https://umtjur.umt.edu.my/wp-content/uploads/sites/141/2021/10/Article-18-UMT-JUR-V3-N4-October-2021.pdf
  1. Fung, F., Wang, H. S., & Menon, S. (2018). Food safety in the 21st century. Biomedical journal, 41(2): 88–95. https://doi.org/10.1016/j.bj.2018.03.003
  1. Correa, T.Q., Blanco, K.C., Garcia, É.B., Perez, S.M.L., Chianfrone, D.J., Morais, V.S., & Bagnato, V.S. (2020) Effects of ultraviolet light and curcumin-mediated photodynamic inactivation on microbial food safety: A study in meat and fruit. Photodiagnosis and Photodynamic Therapy, 30. https://doi.org/10.1016/j.pdpdt.2020.101678
  1. Ruiz-Hernández, K., Ramírez-Rojas, N.Z., Meza-Plaza, E.F. et al. UV-C treatments against Salmonella Typhimurium ATCC 14028 in Inoculated Peanuts and Almonds. Food Engineering Reviews, 13: 706–712 (2021). https://doi.org/10.1007/s12393-020-09272-7
  1. Kim, S. S., Kim, S. H., Park, S. H., & Kang, D. H. (2020). Inactivation of Bacillus cereus Spores on Stainless Steel by Combined Superheated Steam and UV-C Irradiation Treatment. Journal of food protection, 83(1): 13–16. https://doi.org/10.4315/0362-028X.JFP-19-133
  1. Ortali, A., Wright, J., & Onarinde, B. (2021) Effect of UV-C on Escherichia coli, Staphylococcus aureus, Salmonella Typhimurium and SARS-CoV-2 Virus Surrogate (MS2 bacteriophage) Inoculated onto Stainless Steel Surface. World Microbe Forum, 20 – 24 June 2021. https://eprints.lincoln.ac.uk/id/eprint/45210/
  1. Kyere, E.O., Popovich, D.G., Palmer, J., Wargent, J.J., Fletcher, G.C., & Flint, S. (2021) Reductio of the attachment, survival and growth of L. monocytogenes on lettuce leaves by UV-C stress. LWT – Food Science and Technology, 145. https://doi.org/10.1016/j.lwt.2021.111528
  1. Reichel, J., Kehrenberg, C., & Krischek, C. (2019) Inactivation of Yersinia enterocolitica and Brochothrix thermosphacta on pork by UV-C irradiation. Meat Science, 158. https://doi.org/10.1016/j.meatsci.2019.107909
  1. Adhikari, A., Syamaladevi, R. M., Killinger, K., & Sablani, S. S. (2015). Ultraviolet-C light inactivation of Escherichia coli O157:H7 and Listeria monocytogenes on organic fruit surfaces. International Journal of Food Microbiology, 210: 136–142. https://doi.org/10.1016/j.ijfoodmicro.2015.06.018
  1. Ortali, A., Wright, J., & Onarinde, B. (2021) Effect of UV-C on Escherichia coli, Staphylococcus aureus, Salmonella Typhimurium and SARS-CoV-2 Virus Surrogate (MS2 bacteriophage) Inoculated onto Stainless Steel Surface. World Microbe Forum, 20 – 24 June 2021. https://eprints.lincoln.ac.uk/id/eprint/45210/
  1. Ortiz-Solà, J., Valero, A., Abadias, M., Nicolau-Lapeña, I. and Viñas, I. (2022), Evaluation of water-assisted UV-C light and its additive effect with peracetic acid for the inactivation of Listeria monocytogenes, Salmonella enterica and murine norovirus on whole and fresh-cut strawberries during shelf-life. J Sci Food Agric, 102: 5660-5669. https://doi.org/10.1002/jsfa.11913
  1. Ortiz, Jo., Abadias, I., Colás-Medà, P., Anguera, M., & Vinas, I. (2021). Inactivation of Salmonella enterica, Listeria monocytogenes and murine norovirus (MNV-1) on fresh strawberries by conventional and water-assisted ultraviolet light (UV-C). Postharvest Biology and Technology, 174: 111447. https://doi.org/10.1016/j.postharvbio.2020.111447  
  1. Mariita, R.M.; Davis, J.H.; Randive, R.V. (2022) Illuminating Human Norovirus: A Perspective on Disinfection of Water and Surfaces Using UVC, Norovirus Model Organisms, and Radiation Safety Considerations. Pathogens, 11(2): 226. https://doi.org/10.3390/pathogens11020226
  1. de Groot, T., Chowdhary, A., Meis, J.F., & Voss, A. (2019) Killing of Candida auris by UV-C: Importance of exposure time and distance. Mycoses, 65(5): 408-412. https://doi.org/10.1111/myc.12903
  1. Song, H., Dang, Y.M., Ha, S., & Ha, J.H. (2022) Effect of ultraviolet-C light-emitting diode irradiation on inactivation of white colony-forming yeast in kimchi seasoning. Food Control, 140: 101678. https://doi.org/10.1016/j.pdpdt.2020.101678
  1. Vurmaz, A.K., & Gündüz, G.T. (2020). Inhibition of mold growth on the surface of dried persimmons using combined treatments of UV-C light and clove oil. Innovative Food Science and Emerging Technologies, 61: 102336. https://doi.org/10.1016/j.ifset.2020.102336
  1. Scott, C., & Punja, Z. (2020). Evaluation of disease management approaches for powdery mildew on Cannabis sativa L. (marijuana) plants. Canadian Journal of Plant Pathology, 43(3): 394-412. https://doi.org/10.1080/07060661.2020.1836026.
  1. LEDs Magazine. (2023, March 1). SETi and the USDA document UV LEDs extending shelf life of fresh produce. https://www.ledsmagazine.com/horticultural-lighting/agriculture-aquaculture/article/16697306/seti-and-the-usda-document-uv-leds-extending-shelf-life-of-fresh-produce
  1. Stevens, C., Wilson, C.L., Lu, J.Y., Khan, V.A., Chalutz, E., Droby, S., Kabwe, M.K., Haung, Z., Adeyeye, O., Pusey, L.P., Wisniewski, M.E., & West, M. (1996) Plant hormesis induced by ultraviolet light-C for controlling postharvest diseases of tree fruits, Crop Protection, 15(2): 129-134. https://doi.org/10.1016/0261-2194(95)00082-8
  1. Belloli, M., Cigarini, M., Milesi, G., Mutti, P., & Berni, E. (2022) Effectiveness of two UV-C light-emitting diodes (LED) systems in inactivating fungal conidia on polyethylene terephthalate. Innovative Food Science & Emerging Technologies, 79: 103050. https://doi.org/10.1016/j.ifset.2022.103050
  1. Canale, M.C., Benato, E.A., Cia, P. Haddad, M.L., & Pascholati, S.F. (2011) In Vitro effect of UV-C irradiation on Guignardia citricarpa and on postharvest control of citrus black spot. Tropical Plan Pathology, 36(6): 356-361. https://doi.org/10.1590/S1982-56762011000600003  
  1. World Health Organization. (‎2023, March 5)‎. WHO estimates of the global burden of foodborne diseases: foodborne disease burden epidemiology reference group 2007-2015. https://apps.who.int/iris/bitstream/handle/10665/199350/9789241565165_eng.pdf
  1. Murray, K., Wu, F., Shi, J., Xue, S.J., & Warriner, K. (2017) Challenges in the microbiological food safety of fresh produce: Limitations of post-harvest washing and the need for alternative interventions. Food Quality and Safety, 1(4): 289-301. https://doi.org/10.1093/fqsafe/fyx027
  1. Jung, J. & Skinner, K. (2017) Foodborne and waterborne illness among Canadian indigenous populations: a scoping review. Canadian Communicable Disease Report, 43(1): 7-13. https://doi.org/10.14745/ccdr.v43i01a02
  1. Hoffmann, S., & Walter, S.E. (2020) Acute complications and sequelae from foodborne infections: informing priorities for cost of foodborne illness estimates. Foodborne Pathogens Disease, 17(3): 172–177. https://doi.org/10.1089/fpd.2019.2664
  1. Cliver, D. O., & Riemann, H. P. (Eds.). (2002). Foodborne diseases. Gulf Professional Publishing.
  1. Franz, C.M., den Besten, H.M., Boehnlein, C., Gareis, M., Zwietering, M.H., & Fusco, V. (2019) Microbial food safety in the 21st century: Emerging challenges and foodborne pathogenic bacteria. Trends Food Science Technology, 84: 34–37. https://doi.org/10.1016/J.TIFS.2018.09.019
  1. Scallan, E., Hoekstra, R.M., Angulo, F.J., Tauxe, R.V., Widdowson, M.-A., Roy, S.L., Jones, J.L., & Griffin, P.M. (2011) Foodborne illness acquired in the United States—major pathogens. Emerging Infectious Diseases, 17(1), 7–15. https://doi.org/10.3201/eid1701.P11101
  1. Kirk, M.D., Pires, S.M., Black, R.E., Caipo, M., Crump, J.A., Devleesschauwer, B., Dopfer, D., Fazil, A., Fischer-Walker, C.L., Hald, T., et al. (2015) World Health Organization estimates of the global and regional disease burden of 22 foodborne bacterial, protozoal, and viral diseases, 2010: A data synthesis. PLoS Medicine, 12(12): e1001921. https://doi.org/10.1371/journal.pmed.1001921
  1. Kim, S. S., Kim, S. H., Park, S. H., & Kang, D. H. (2020). Inactivation of Bacillus cereus Spores on Stainless Steel by Combined Superheated Steam and UV-C Irradiation Treatment. Journal of food protection, 83(1): 13–16. https://doi.org/10.4315/0362-028X.JFP-19-133
  1. Hinds, L.M., O’Donnell, C.P., Akhter, M., & Tiwari, B.K. (2019) Principles and mechanisms of ultraviolet light emitting diode technology for food industry applications. Innovative Food Science & Emerging Technologies, 56: 102153. https://doi.org/10.1016/j.ifset.2019.04.006  
  1. Oniciuc, E.A., Likotrafiti, E., Alvarez-Molina, A., Prieto, M., Lopez, M., & Alvarez-Ordonez, A. (2019) Food processing as a risk factor for antimicrobial resistance spread along the food chain. Current Opinion in Food Science, 30: 21-26. https://doi.org/10/1016/j.cofs.2018.09.002    
  1. Singh, H., Bhardwaj, S.K., Khatri, M., Kim, K.H., & Bhardwaj, N. (2021) UVC radiation for food safety: An emerging technology for the microbial disinfection of food products. Chemical Engineering Journal, 417: 128084. https://doi.org/10.1016/j.cej.2020.128084   
  1. Hinds, L.M., O’Donnell, C.P., Akhter, M., & Tiwari, B.K. (2019) Principles and mechanisms of ultraviolet light emitting diode technology for food industry applications. Innovative Food Science & Emerging Technologies, 56: 102153. https://doi.org/10.1016/j.ifset.2019.04.006
  1. Kowalski, W.J. (2009) Ultraviolet Germicidal Irradiation Handbook: UVGI for Air and Surface Disinfection. Springer, New York.
  1. Hinds, L.M., O’Donnell, C.P., Akhter, M., & Tiwari, B.K. (2019) Principles and mechanisms of ultraviolet light emitting diode technology for food industry applications. Innovative Food Science & Emerging Technologies, 56: 102153. https://doi.org/10.1016/j.ifset.2019.04.006
  1. Hinds, L.M., O’Donnell, C.P., Akhter, M., & Tiwari, B.K. (2019) Principles and mechanisms of ultraviolet light emitting diode technology for food industry applications. Innovative Food Science & Emerging Technologies, 56: 102153. https://doi.org/10.1016/j.ifset.2019.04.006
  1. Crystal IS. (2023, March 5). Rethinking UVC LED Lifetime Requirement for Disinfection Using Operating Hours. https://klaran.com/rethinking-uvc-led-lifetime-requirements-for-disinfection-using-operating-hours
  1. Hinds, L.M., O’Donnell, C.P., Akhter, M., & Tiwari, B.K. (2019) Principles and mechanisms of ultraviolet light emitting diode technology for food industry applications. Innovative Food Science & Emerging Technologies, 56: 102153. https://doi.org/10.1016/j.ifset.2019.04.006
  1. Mariita, R.M., Davis, J.H., & Randive, R.V. (2022) Illuminating Human Norovirus: A Perspective on Disinfection of Water and Surfaces Using UVC, Norovirus Model Organisms, and Radiation Safety Considerations. Pathogens, 11(2), 226. https://doi.org/10.3390/pathogens11020226
  1. Marinez-Sanchez, A., Lozano-Pastor, P., Artes-Hernandez, F., Artes, F., & Aguayo, E. (2019) Preharvest UV-C treatment improves the quality of spinach primary production and postharvest storage. Postharvest Biology and Technology, 155: 130-139. https://doi.org/10.1016/j.postharvbio.2019.05.021   
  1. Nicolau-Lapena, I., Abadias, M., Vinas, I., Bobo, G., Lafarga, T., Ribas-Afusti, A., & Aguilo-Aguayo, I. (2020) Water UV-C treatment alone or in combination with peracetic acid: A technology to maintain safety and quality of strawberries. International Journal of Food Microbiology, 335: 108887. https://doi.org/10.1016/j.ijfoodmicro.2020.108887
  1. Ortiz, Jo., Abadias, I., Colás-Medà, P., Anguera, M., & Vinas, I. (2021). Inactivation of Salmonella enterica, Listeria monocytogenes and murine norovirus (MNV-1) on fresh strawberries by conventional and water-assisted ultraviolet light (UV-C). Postharvest Biology and Technology, 174: 111447. https://doi.org/10.1016/j.postharvbio.2020.111447
  1. Hosseini, F.-S., Akhavan, H.-R., Maghsoudi, H., Hajimohammadi-Farimani, R., & Balvardi, M. (2019), Effects of a rotational UV-C irradiation system and packaging on the shelf life of fresh pistachio. Journal of the Science of Food and Agriculture, 99(11): 5229-5238. https://doi.org/10.1002/jsfa.9763
  1. Kim, H-J., Lee, C.-L., Yoon, K.-S., & Rhim, J.-W. (2022) Synergistic effect of UV-C LED irradiation and PLA/PBAT-based antimicrobial packaging film on fresh-cut vegetables. Food Control, 138: 109027. https://doi.org/10.1016/j.foodcont.2022.109027 
  1. Marinez-Sanchez, A., Lozano-Pastor, P., Artes-Hernandez, F., Artes, F., & Aguayo, E. (2019) Preharvest UV-C treatment improves the quality of spinach primary production and postharvest storage. Postharvest Biology and Technology, 155: 130-139. https://doi.org/10.1016/j.postharvbio.2019.05.021   
  1. Hemmaty, S., Moallemi, N., & Naseri L. (2006) Shelf-life and quality of apple fruits in response to posthartvest application of UV-C radiation. Journal of Applied Horticulture, 8(2): 114-116. https://www.horticultureresearch.net/jah/2006_8_2_114_116.PDF
  1. Finardi, S., Hoffmann, T.G., Schmitz, F.R.W, Bertoli, S.L., Khayrullin, M., Neverova, O., Ponomarev, E., Goncharov, A., Kulmakova, N., Dotsenko, E., Khryuchkina, E., Shariati, M.A., & de Souza, C.K. (2021) Comprehensive Study of Light-Emitting Dioldes (LEDs) and Ultraviolet-LED lights Application in Food Quality and Safety. Journal of Pure and Applied Microbiology, 15(3): 1125-1135. https://doi.org/10.22207/JPAM.15.3.54
  1. Zhang, X., Zhang, M., Xu, B., Mujumdar, A.S., & Guo, Z. (2021) Light-emitting diodes (below 700 nm): Improving the preservation of fresh foods during postharvest handling, storage, and transportation. Comprehensive Reviews in Food Science and Food Safety, 21(1): 106-126. https://doi.org/10.1111/1541.4337.12887
  1. Calle, A., Fernandez, M., Montoya, B., Schmidt, M., & Thompson, J. (2021) UV-C LED Irradiation Reduces Salmonella on Chicken and Food Contact Surfaces. Foods, 10(7): 1459. https://doi.org/10.3390/foods10071459
  1. Kim, H-J., Lee, C.-L., Yoon, K.-S., & Rhim, J.-W. (2022) Synergistic effect of UV-C LED irradiation and PLA/PBAT-based antimicrobial packaging film on fresh-cut vegetables. Food Control, 138: 109027. https://doi.org/10.1016/j.foodcont.2022.109027
  1. Kyere, E.O., Popovich, D.G., Palmer, J., Wargent, J.J., Fletcher, G.C., & Flint, S. (2021) Reductio of the attachment, survival and growth of L. monocytogenes on lettuce leaves by UV-C stress. LWT – Food Science and Technology, 145. https://doi.org/10.1016/j.lwt.2021.111528
  1. Vurmaz, A.K., & Gündüz, G.T. (2020). Inhibition of mold growth on the surface of dried persimmons using combined treatments of UV-C light and clove oil. Innovative Food Science and Emerging Technologies, 61: 102336. https://doi.org/10.1016/j.ifset.2020.102336
  1. Deng, L.-Z., Tao, Y., Mujumdar, A.S., Pan, Z., Chen, C., Yang, X.-H., Liu, Z.-L., Wang, H., & Xiao, H.-W. (2020) Recent advances in non-thermal decontamination technologies for microorganisms and mycotoxins in low-moisture foods. Trends in Food Science & Technology, 106: 104-112. https://doi.org/10.1016/j.tifs.2020.10.012
  1. Singh, H., Bhardwaj, S.K., Khatri, M., Kim, K.H., & Bhardwaj, N. (2021) UVC radiation for food safety: An emerging technology for the microbial disinfection of food products. Chemical Engineering Journal, 417: 128084. https://doi.org/10.1016/j.cej.2020.128084
  1. Correa, T.Q., Blanco, K.C., Garcia, É.B., Perez, S.M.L., Chianfrone, D.J., Morais, V.S., & Bagnato, V.S. (2020) Effects of ultraviolet light and curcumin-mediated photodynamic inactivation on microbial food safety: A study in meat and fruit. Photodiagnosis and Photodynamic Therapy, 30. https://doi.org/10.1016/j.pdpdt.2020.101678
  1. Reichel, J., Kehrenberg, C., & Krischek, C. (2019) Inactivation of Yersinia enterocolitica and Brochothrix thermosphacta on pork by UV-C irradiation. Meat Science, 158. https://doi.org/10.1016/j.meatsci.2019.107909
  1. Monteiro, M.L.G., Mársico, E.T., Mutz, Y.d.S. et al. (2020) Combined effect of oxygen-scavenger packaging and UV-C radiation on shelf life of refrigerated tilapia (Oreochromis niloticus) fillets. Scientific Reports, 10: 4243. https://doi.org/10.1038/s41598-020-61293-8
  1. Darré, M., Vicente, A. R., Cisneros-Zevallos, L., & Artés-Hernández, F. (2022). Postharvest Ultraviolet Radiation in Fruit and Vegetables: Applications and Factors Modulating Its Efficacy on Bioactive Compounds and Microbial Growth. Foods (Basel, Switzerland), 11(5), 653. https://doi.org/10.3390/foods11050653
  1. Malek, N. I., Wan Abdullah, W. Z. ., & Wan Sembok, W. Z. . (2021). Effects of UV-C Radition in Delaying of Ripening of Berangan Banana (Musa sp. AAA Berangan). Universiti Malaysia Terengganu Journal of Undergraduate Research, 3(4), 173–182. https://doi.org/10.46754/umtjur.v3i4.250
  1. Ortali, A., Wright, J., & Onarinde, B. (2021) Effect of UV-C on Escherichia coli, Staphylococcus aureus, Salmonella Typhimurium and SARS-CoV-2 Virus Surrogate (MS2 bacteriophage) Inoculated onto Stainless Steel Surface. World Microbe Forum, 20 – 24 June 2021. https://eprints.lincoln.ac.uk/id/eprint/45210/
  1. Kim, S. S., Kim, S. H., Park, S. H., & Kang, D. H. (2020). Inactivation of Bacillus cereus Spores on Stainless Steel by Combined Superheated Steam and UV-C Irradiation Treatment. Journal of food protection, 83(1): 13–16. https://doi.org/10.4315/0362-028X.JFP-19-133
  1. Mariita, R.M., Davis, J.H., Randive, R.V. (2022) Illuminating Human Norovirus: A Perspective on Disinfection of Water and Surfaces Using UVC, Norovirus Model Organisms, and Radiation Safety Considerations. Pathogens, 11(2): 226. https://doi.org/10.3390/pathogens11020226
  1. Dai, T., Vrahas, M.S., Murray, C.K., Hamblin, M.R. (2012) Ultraviolet C Irradiation: An Alternative Antimicrobial Approach to Localized Infections? Expert Review of Anti-infective Therapy, 10(2): 185-195. https://doi.org/10.1586/eri.11.166
  1. Finsen, N.R. (1901) Phototherapy (translated to English by J.H. Sequeira). Edward Arnold Press, London.
  1. Barnard, J.E., Morgan, H. de R. (1903) The Physical Factors in Phototherapy. British Medical Journal, 2(2237): 1269-1271. https://doi.org/10.1136/bmj.2.2237.1269.
  1. Reed, N.G. (1974) The history of ultraviolet germicidal irradiation for air disinfection. Public health reports, 125(1): 15-27. 
  1. Koo, M., Park, S.Y., Lee, J. (2012). Biointegrated flexible inorganic light emitting diodes. Nanobiosensors in Disease Diagnosis, 1: 5-15. https://doi.org/10.2147/NDD.S26593.
  1. Skinner, N.P., Bullough, J.D., Rea, M.S. (2021) Science Enlightening Agriculture: Helping Growers Improve Crops with UV Light. https://cfgrower.com/science-enlightening-agriculture-helping-growers-improve-crops-with-uv-light/.
  1. Morey, A., McKee, S.R., Dickson, J.S., Singh, M. (2010) Efficacy of Ultraviolet Light Exposure Against Survival of Listeria monocytogenes on Conveyor Belts. Foodborne Pathogens and Disease, 7(6): 737-740. https://doi.org/10.1089/fpd.2009.0464.
  1. Cassar, J.R., Mills, E.W., Demirci, A. (2022) Pulsed Ultraviolet Light Decontamination of Meat Conveyor Surfaces. Food Science and Technology International, 28(8): 663-671. https://doi.org/10.1177/10820132211049610.
  1. Abrams, A. (2018). These Are the Dirtiest Places in Your Hotel Room. Retrieved from http://time.com/5135121/dirty- hotel-rooms-germs/
  1. Sifuentes, L.Y., Koenig, D.W., Phillips, R.L., Reynolds, K.A., Gerba, C.P. (2014) Use of Hygiene Protocols to Control the Spread of Viruses in a Hotel. Food and Environmental Virology, 6(3): 175-81. https://doi.org/10.1007/s12560-014-9158-0.
  1. Dancer, S.J. (2014) Controlling Hospital-Acquired Infection: Focus on the Role of the Environment and New Technologies for Decontamination. Clinical Microbiology Reviews, 27(4): 665-690. https://doi.org/10.1128/cmr.00020-14.ht.
  1. Rutala, W., Kanamori, H., Gergen, M., Sickbert-Bennett, E., Knelson, L., Chen, L.F., Sexton, D.J., Anderson, D., Weber, D.J. (2016) Microbial Load on Environmental Surfaces: The Relationship Between Reduced Environmental Contamination and Reduction of Healthcare-Associated Infections. Open Forum Infectious Diseases, 3(1). https://doi.org/10.1093/ofid/ofw172.128.